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Fig. 5 | Reproductive Biology and Endocrinology

Fig. 5

From: Homozygous mutation in SLO3 leads to severe asthenoteratozoospermia due to acrosome hypoplasia and mitochondrial sheath malformations

Fig. 5

Effects of SLO3 deficiency on mitochondrial function. A Influence of the SLO3 mutation on mitochondrial membrane potential (MMP). Assessment of mitochondrial membrane potential by flow cytometry acquisition for JC-1-stained (a marker of MMP) cells was performed through FL1 for green and FL2 for red fluorescence. MMP is significantly reduced in mutant sperm compared with control. B Immunoblotting of sperm lysates from controls and the SLO3-mutated individual using the anti-HSP60 antibody. β-actin was used as loading control. C The expression of HSP60 is almost diminished in SLO3 mutant spermatozoa. HSP60 staining is present in the flagellar midpiece of spermatozoa from normal controls, but significantly reduced in spermatozoa from A-132. Scale bars, 20 μm

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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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