Fig. 3From: Expressions of VEGF-A and VEGFR-2 in placentae from GDM pregnanciesExpressions of VEGFA and VEGFR2 mRNAs and proteins in GDM (nā=ā20) and control (nā=ā20) placental tissues by real-time PCR and Western immunoblotting. Relative quantification of VEGFA (a) and VEGFR2 (b) mRNA expression normalized to the expression of ACTIN in all the samples. Data were analyzed according to the 2āĪĪCT method. A representative immunoblots for VEGFA (c) and VEGFR2 (d) were shown. Immumoblots representing Ī²-tubulin protein (middle panels) showed the loading equally total protein (25Ā Ī¼g). Semi-quantitative analyses of VEGFA and VEGFR2 immunoreactive proteins were performed relative to Ī²-tubulin (bottom panels). The Y-axis represents the expression levels of VEGFA and VEGFR2 related to actin or tubulin. *Pā<ā0.05, **Pā<ā0.01, Studentās t testBack to article page