Fig. 5
From: 17β-estradiol modifies human spermatozoa mitochondrial function in vitro
Changes of mitochondrial superoxide anion level (using MitoSOX Red dye) after 17β-estradiol stimulation. a Percentage of MitoSOX-positive sperm treated with E2. b Significant dose-dependent increase in intensity of MitoSOX fluorescence after E2 stimulation. Data obtained from 10 separate analyses are expressed as means ± standard deviation. Different superscript letters above each bar denote significant difference, P < 0.05