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Table 2 Primer pairs for amplification of reference genes

From: mRNA expression pattern of selected candidate genes differs in bovine oviductal epithelial cells in vitro compared with the in vivo state and during cell culture passages

Gene

Sequence of nucleotide

Accession no. /Reference

Product size (bp)

Tm (°C)

HDAC1

for 5′ –CCA GTG CAG TTG TCT TGC AG- 3′

NM_001037444.2

217

60

rev 5′ –TTA GGG ATC TCC GTG TCC AG- 3′

UXT

for 5′ –CGC TAC GAG GCT TTC ATC TC- 3′

NM_001037471.2

207

61

rev 5′ –TGA AGT GTC TGG GAC CAC TG- 3′

PPIA

for 5′ – CTG AGC ACT GGA GAG AAA GG- 3′

NM_178320.2

259

60

rev 5′ – TGC CAT CCA ACC ACT CAG TC- 3′

RPL19

for 5′ -GGC AGG CAT ATG GGT ATA GG- 3′

NM_001040516.1

232

60

rev 5′ -CCT TGT CTG CCT TCA GCT TG- 3′

SUZ12

for 5′ -TTC GTT GGA CAG GAG AGA CC- 3′

[59]

286

60

rev 5′ -GTG CAC CAA GGG CAA TGT AG- 3′

  1. Selected gene transcripts, primer sequences and annealing temperatures (Tm) used for normalization of quantitative PCR with resulting amplicon length