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Fig. 3 | Reproductive Biology and Endocrinology

Fig. 3

From: Divergent activity of the gonadotropin-releasing hormone receptor gene promoter among genetic lines of pigs is partially conferred by nuclear factor (NF)-kB, specificity protein (SP)1-like and GATA-4 binding sites

Fig. 3

Divergence of GnRHR promoter activity between lines is not alone conferred by the −1915/−1431 bp region. Transient transfection of αT3-1 cells with GnRHR gene promoter “swap” luciferase (LUC) reporter constructs for Meishan and Control lines of swine was performed. The −M(C)5118pGL3 vector is the full-length Meishan reporter construct with the 484 bp region from −1915 to −1431 bp replaced with the Control promoter sequence from that region, whereas the −C(M)5118pGL3 vector is the reverse. Unique letters indicate means different from one another (P < 0.05)

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