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Fig. 2 | Reproductive Biology and Endocrinology

Fig. 2

From: Divergent activity of the gonadotropin-releasing hormone receptor gene promoter among genetic lines of pigs is partially conferred by nuclear factor (NF)-kB, specificity protein (SP)1-like and GATA-4 binding sites

Fig. 2

A SF1 binding site is crucial for transcriptional activity of the GnRHR gene in all three genetic lines of swine. Luciferase (LUC) reporter vectors containing block replacement mutation of the SF1 binding site in the context of the full-length promoter were constructed for all three lines of swine (Meishan, −MμSF1pGL3; Index, −IμSF1pGL3; Control, −CμSF1pGL3). These vectors, along with vectors containing full-length native GnRHR promoter for Meishan (−M5118pGL3), Index (−I5118pGL3) and Control (−C5118pGL3) lines of swine were transiently transfected into αT3-1 cells. An asterisk indicates means that are greater than promoterless control (pGL3; P < 0.05)

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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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