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Fig. 1 | Reproductive Biology and Endocrinology

Fig. 1

From: Divergent activity of the gonadotropin-releasing hormone receptor gene promoter among genetic lines of pigs is partially conferred by nuclear factor (NF)-kB, specificity protein (SP)1-like and GATA-4 binding sites

Fig. 1

Reporter constructs containing GnRHR promoters from Meishan, Index and Control swine lines exhibit divergent activity. αT3-1 cells were transiently transfected with luciferase (LUC) reporter vectors containing either 5118 or 1915 bp of GnRHR promoter for the Meishan (−M5118pGL3 and −M1915pGL3), Index (−I5118pGL3 and −I1915pGL3) and Control (−C5118pGL3 and −C1915pGL3) lines of swine, 600 bp of the mouse GnRHR promoter (−m600pGL3), or promoterless control (pGL3). The −m600pGL3 vector was included as a positive control because it has been shown to have robust activity in this cell line. Unique letters within vectors containing the same portion of 5’ flanking sequence (−5118 or −1915) for each swine line (brackets) indicate values that are significantly different from one another (P < 0.05). An asterisk indicates a mean significantly different from all others (P < 0.05)

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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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