Table 1 Parameters of the applied primers and PCR reactions. The real-time reverse transcription polymerase chain reactions were used to determine the changes in the mRNA expression. In our studies the parameters of inventoried TaqMan assays were defined by Life Technologies (ThermoFisher Scientific, Hungary)
TaqMan assays | Assay ID (Life Technologies, Hungary) | Accession number | Assay location | Amplicon length | Annealing temp. (°C) | Reaction volume (μl) |
|---|---|---|---|---|---|---|
α2A-AR | Rn00562488_s1 | NM_012739.3 | 1350 | 72 | 60 | 20 |
α2B-AR | Rn00593312_s1 | NM_138505.2 | 1451 | 63 | 60 | 20 |
α2C-AR | Rn00593341_s1 | NM_138506.1 | 653 | 111 | 60 | 20 |
β-actin | Rn00667869_m1 | NM_031144.3 | 881 | 91 | 60 | 20 |