Fig. 4
From: Post-thaw ATP supplementation enhances cryoprotective effect of iodixanol in rat spermatozoa
The effect of OptiPrep™ and ATP treatment on post-thaw AMI and MMP of rat spermatozoa. Frozen-thawed spermatozoa were stained with PNA-Alexa 488/PI (a) and the percentages of PNA−/PI− (b), PNA+/PI− (c) and PNA+/PI+ (d) were analyzed with flow cytometry. Using JC-1/PI staining, viable spermatozoa with high MMP (e), Mean Jagg fluorescence intensity (Jagg MFI) per viable sperm (f), ratio of spermatozoa with high MMP of viable spermatozoa (g) were evaluated for MMP. PNA−/PI−: viable and acrosome-intact, PNA+/PI−: viable and acrosome-reacted, PNA+/PI+: dead and acrosome-reacted/or damaged, PNA−/PI+: dead, PNA+: total acrosome-reacted/or damaged. *P < 0.05 v.s. ATP-free within OptiPrep™ groups (comparison between ATP-free and ATP-supplemented groups). n = 6