Table 1 List of primers used for cloning and quantitative RT-PCR.
Step | Gene name | Strand | Sequence | Product size (bp) |
|---|---|---|---|---|
cloning | cx30.9 | Sense | 5'-GAAATTCCGAGGAGGTGGTC-3' | 1,088 |
| Â | Â | Antisense | 5'-GCATGTTTGTCCAGGACTAC-3' | Â |
cloning | cx34.3 | Sense | 5'-CTGAGAGACTGAGAGCTCCAAAC-3' | 1,038 |
| Â | Â | Antisense | 5'-AGACTGGCATGCGAATCATTG-3' | Â |
cloning | cx43.2 | Sense | 5'-TCAAGAAGTGACGGAGAAAG-3' | 1,278 |
| Â | Â | Antisense | 5'-GTACTCTCTGTTCTCTGGCA-3' | Â |
cloning | cx44.9 | Sense | 5'-AGCCTTAAAGAGCTGAGAGG-3' | 1,273 |
| Â | Â | Antisense | 5'-CGAGCAGGCATTAAGCAGGA-3' | Â |
qPCR* | cx30.9 | Antisense | 5'-GGCTGGTGGAGTGTTTGTTC-3' | 99 |
qPCR | cx34.3 | Sense | 5'-ACTACCTGTATGGCTTCACCCT-3' | 284 |
| Â | Â | Antisense | 5'-CTGGATCATCTGGTCTTTGTTC-3' | Â |
qPCR | cx43.2 | Sense | 5'-ATGGCTGTTCCTCTCCCACT-3' | 121 |
| Â | Â | Antisense | 5'-CAGTTTTGCTCGTTGGCTTG-3' | Â |
qPCR | cx44.9 | Sense | 5'-GTGACAGAAGGCTCTGTGAAG-3' | 305 |
| Â | Â | Antisense | 5'-ACACCGAGATGGAGAATCTC-3' | Â |