Stages | Classification | Macroscopic appearance | Oocyte size (μm) | GSI range | ||
---|---|---|---|---|---|---|
 |  | Testes | Ovary |  | F | M |
I | Immature | Small, flat, translucent to whitish, poorly developed, with reduced fringes. | Small, transparent to translucent and not very voluminous. Oocyte not visible with naked eye. | 209.61-592.78 (447.30 ± 99.42)a | <10.9 | <2.0 |
II | Develop | Whitish with voluminous fringes. | Large orange-pale, oocytes may be visible through the ovary tunic. | 528.37-867.10 (711.24 ± 63.21)b | 11.0-18.9 | 2.5-5.5 |
III | Mature | Very large, firm, white in colour. | Very large occupying part of the abdominal cavity. Yellow oocyte turgescency. | 604.15-894.33 (780.59 ± 50.71)c | 19.0-23.9 | 5.6-8.0 |
IV | Ripe | Full developed, turgid fringes, milky-whitish in colour. Milt run out of the fish. | Occupying the entire abdominal cavity. Ovulated oocytes can be fully expelled from the oviduct with gentle pressure. | 725.27-991.81 (844.09 ± 57.59)d | >24.0 | > 8.0 |
V | Spent | Bloody and flaccid fringes. | Flaccid, red-brown or bloody in colour. Few remaining large oocytes observed, and smaller size oocytes may be seen. | 227.64-770.82 (447.38 ± 139.02)a | 2.5-10.9 | 1.5-3.0 |