Figure 1

Localization of ERp29 on BALB/c mouse spermatozoa by indirect immunofluorescence analysis. (A), (E), (I) The localization of ERp29 on spermatozoa from epididymal caput, caudal regions and on acrosome-reacted spermatozoa. (B), (F), (J) Differential interference contrast (DIC) images corresponding to (A), (E), (I). (C),(G),(K) Caput, caudal and acrosome-reacted spermatozoa incubated with normal rabbit IgG as negative controls. (D), (H), (L) DIC images corresponding to (C), (G), (K). (M),(N) Caput sperm head in (A),(B) were magnified to show the precise localization. (P),(Q) Caudal sperm head in (E),(F) were magnified to show the precise localization. (S),(T) Acrosome-reacted sperm head in (I),(J) were magnified to show the precise localization. (O),(R),(U) The combined image of (M) and (N), (P) and (Q), (S) and (T), respectively. The arrows indicated the exact localization of ERp29 in the spermatozoa on different conditions. Calibration bar = 5 μm.