Figure 4

We cotransfected JEG3 cells expressing CRE-luciferase and β-galactosidase expression vectors with empty vector (0.5 μg) and DN-MyD88 (0.1, 0.3, 0.5 μg) or DN-TRIF (0.1, 0.3, 0.5 μg) cDNA as described above, treated them with cAMP (0.5 μM) or media for 5 h, and assessed the luciferase activity. Calorimetric β-galactosidase assay was performed to correct for the transfection efficiency. (*p < 0.01 compared the cAMP treated empty vector transfected control). Luciferase activity was expressed as relative light unit (RLU). Each experiment was performed in triplicate or quadruplicate and repeated at least three independent times.