Figure 3
The CYP17 silenced by lentivirus shRNAs in vitro. A, GFP fluorescence observed 72 hours after lentivirus transduction. B, Quantitative assay of CYP17 mRNA levels 5 days after lentivirus transduction from six different replicates. C, CYP17 protein levels 7 days after lentivirus transduction. D, Quantitative results of Western blotting images from three different replicates. CON: Blank Control; NC: Negative Control: 1#: CYP17 shRNA1#; 2#: CYP17 shRNA2#; 3#: CYP17 shRNA3#. ANOVA, *P < 0.05 when compared with CON and NC groups. *ANOVA, P < 0.05, when compared with both CON and NC groups. CON: Blank Control; NC: Negative Control: 2#: CYP17 shRNA2#.