From: Red blood cell glutathione peroxidase activity in female nulligravid and pregnant rats
Content | Concentration |
---|---|
Potassium phosphate, containing 2,5 mM Na2EDTA, 2,5 mM sodium azide, pH 7.0 | 0,5 M |
Glutathione reductase (from baker yeast, ammonium sulfate suspension, 100–300 U/mg protein) in the same phosphate buffer; | 0,18 U/ml: at 25°C |
GSH (>98%) in distilled water | 100 mM |
NADPH (~95%) in 0,1% NaHCO3 solution | 10 mM |
Cumene hydroperoxide (>80%) in distilled water | 60 mM |