Table 1 Used antibodies, suppliers, antigen retrieval and dilutions.

Primary antibodies

PCNA

PC-10. Novocastra (UK)

Buffer Citrate pH 6. Microwave.

1:100

Ki-67

MIB-5. Dako (Denmark)

Buffer Citrate pH 6. Microwave.

1:60

Active Caspase-3

Polyclonal. R&D systems (USA)

Without antigen retrieval

1:500

Bax

Polyclonal. PU347-UP-Biogenex (San Ramon, CA, USA)

Without antigen retrieval

1:30

Bcl-2

Clone 100. Zymed (San Francisco, CA, USA)

Without antigen retrieval

1:50

Bcl-w

Polyclonal. ab 13525-Abcam (Cambridge, UK)

Without antigen retrieval

1:100

Bcl-xL

Polyclonal. ab 45002-Abcam (Cambridge, UK)

Without antigen retrieval

1:500

Secondary antibodies

Anti-rabbit IgG

Goat Polyclonal. 65-6140-Zymed (San Francisco, CA, USA)

-

1:100

Anti-mouse IgG

Goat Polyclonal. AP181B Chemicon (Temecula, CA, USA)

-

1:100