Figure 4

Immunoblot analysis of transient expression of wildtype (wt), dominant negative (dn), and constitutively active (ca) EGFP-ILK fusion proteins in BeWo cells. A representative immunoblot demonstrating that the EGFP-ILK fusion proteins were readily detectable in BeWo cells 48 h post-transfection and absent in the empty vector transfected cells (pEGFP). Endogenous ILK (ILK) was detectable in all transfected and non-transfected cell lysates and total ERK2 detection served as an indication of comparable protein loading between lanes.