Figure 1

A) Binding of ³H-progesterone (cpm) was measured in increasing amounts of crude membranes from CHO cells and CHO cells expressing mPRα. Displacement of ³H-progesterone was measured in the presence of 1000-fold excess nonradioactive progesterone. Values are a representation of three individual experiments. B) Binding of ³H-Progesterone in crude membrane fractions from CHO cells expressing the ovine mPRα at various times of incubation. Displacement of ³H-progesterone was measured in the presence of 1000-fold excess nonradioactive progesterone. Values are means ± S.E. (n = 4).