Figure 5
From: A novel promoter controls Cyp19a1 gene expression in mouse adipose tissue
Regulation of the adipose-specific promoter of the Cyp19a1 gene by dexamethasone (A) and Bt2cAMP plus PDA (B). Luciferase plasmids containing the 5'-flanking promoter region of the mouse Cyp19a1 gene in gonadal fat (-343/-1 bp and -1637/-1 bp) were transfected into murine 3T3 L1 cells for 24 hours. pGL4.74 [hRluc] Renilla was used as an internal control for transfection efficiency. Promoter activity was normalized to pGL4.74 [hRluc] Renilla and was represented as the average of data from triplicate replicates; the empty luciferase vector PGL4.10 [luc2] without treatment was arbitrarily assigned a unit of 1, and results are expressed as multiples of the PGL4.10 [luc2] vector. All results are given as mean ± SE from at least 3 independent experiments performed in triplicate. *p < 0.05 for vs. the empty luciferase vector PGL4.10 [luc2] without treatment. †p < 0.05 for vs. vehicle treatment.