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Figure 3 | Reproductive Biology and Endocrinology

Figure 3

From: Absence of spermatozoal CD46 protein expression and associated rapid acrosome reaction rate in striped field mice (Apodemus agrarius)

Figure 3

A. agrarius epididymal spermatozoa fail to express CD46 protein. Immunostaining for CD46 (green) in the acrosomal region of epididymal sperm from M. m. musculus (A and C) and A. agrarius (B and D) using an affinity-purified rabbit polyclonal antibody against A. sylvaticus CD46 SCR1 peptide (A and B) or a rat polyclonal antiserum against rat CD46 (C and D). Phase contrast micrographs showing the head morphology of M. m. musculus (E) and A. agrarius (F) sperm. Nuclei are counterstained with DAPI (blue). Scale bars represent 10 μm.

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