Identification and characterization of Ca2+-activated K+ channels in granulosa cells of the human ovary

Table 1 Oligodeoxynucleotide primer pairs used for PCR amplification.

Channel subunit	Alternative nomenclature	Primer sequence 5' - 3'	GenBank accession no.	Annealing temperature	Product size	Source
BK β1	KCNMB1	Sense AAGGTCAGAGCCAAATTCCAAG	NM_004137	57°C	80 bp	ID 4758626a2
		Antisense AATAGGACGCTGGTTTCGTTC
		Sense AGGAGGAGCTGAAGGGCAAGAAGG		57°C	309 bp	Lasergene
		Antisense AGGTGGGCCAGAAGAGGGAGAAGA
BK β2	KCNMB2	Sense AATCACACTCCTGCGCTCATACAT	NM_181361	59°C	177 bp	Lasergene
		Antisense TCCCCGGAAGAAGTCAGGTTA
BK β3	KCNMB3	Sense TTGCTCGGAACAACCATTCTAAA	NM_171829	57°C	155 bp	ID 25952102a2
		Antisense AGACACGGGTACTTCCCCTG
BK β4	KCNMB4	Sense GGAAAGATGAGATTGGTTCCCAG	NM_014505	57°C	160 bp	ID 26051275a3
		Antisense AGGACCACAATGAGAACGCC
SK1	KCNN1	Sense AGACGTGGCTCATCTACAAACA	NM_002248	59°C	149 bp	ID 25777643a3
		Antisense CTGGTCGTTCAGCTTCCCTT
SK2	KCNN2	Sense CAAGCAAACACTTTGGTGGA	NM_021614	55°C	451 bp	[38]
		Antisense TGTTCAGGTTCCCAGGATTC
SK3	KCNN3	Sense CATGTTTTCGTTGGCCCTGAA	NM_002249	57°C	146 bp	ID 25777650a2
		Antisense GCTCGTAGGTCATGGCTATCC
IK	KCNN4	Sense TGTTCTACAAACATACTCGCAGG	NM_002250	64°C	134 bp	Lasergene
	SK4, K_Ca3.1	Antisense CATGGAGTTCACTTGTTCCCG

Primer sequences were derived from the PrimerBank (ID; http://pga.mgh.harvard.edu/primerbank; [37], from the literature, or designed using the software Lasergene (DNAStar; see source).

ISSN: 1477-7827