Figure 2From: Leukocytospermia and sperm preparation - a flow cytometric studyPre- (2a) and post-processing (2b, 2c) cytofluorimetric analysis of a semen sample with a low leukocyte concentration. Cytogram 2a displays the cytofluorimetric analysis of a semen sample containing a low concentration of leukocytes, stained with Syto-16 and CD45 to identify sperm and leukocytes. Region R2 (blue colour) and Region 3 (red colour) represent the number of leukocytes and sperm counted, respectively. Cytograms 2b and 2c displays the cytofluorimetric analysis of the same sample after density-gradient centrifugation and after swim-up technique, respectively.Back to article page