Table 1 Primer sequences, gene accession number and Q-PCR conditions.
mRNA target | GenBank accession No. | Primers | Conditions | Ref. |
|---|---|---|---|---|
β-actin |  |  | 95°C, 15 sec | Cintado et al., 2001 |
|  | NM_031144 | Sense 5'-CCTAGCACCATGAAGATCAA-3' | 60°C, 20 sec |  |
|  |  | Antisense 5'-TTTCTGCGCAAGTTAGGTTTT-3' | 72°C, 30 sec |  |
Cb1 | NM_012784 |  | 95°C, 20 sec | Porcella et al., 1998 |
|  |  | Sense 5'-CATCATCATCCACACGTCAG-3' | 60°C, 30 sec |  |
|  |  | Antisense 5'-ATGCTGTTGTCTAGAGGCTG-3' | 72°C, 20 sec |  |
Cb2 | NM_020543 |  | 95°C, 20 sec | Porcella et al., 1998 |
|  |  | Sense 5'-TTTCCCACTGATCCCTAACG-3' | 60°C, 30 sec |  |
|  |  | Antisense 5'-AGTTAACAAGGCACAGCATG-3' | 72°C, 20 sec |  |
Trpv1 | AF237067 |  | 95°C, 25 sec | Dvorakova et al., 2001 |
|  |  | Sense 5'-TGGAACAACGGGCTAGCTTA-3' | 58°C, 30 sec |  |
|  |  | Antisense 5'-TCCTCATAAGGGCAGTCCAG-3' | 72°C, 25 sec |  |