Figure 4

Primary culture of Sertoli cells from EGFP transgenic mice (FM131) transfected with pGtoR. The analysis was performed at 120 h (A, B and C) and 140 h (D, E and F) after transfection. Green fluorescence (excitation wavelength 488 nm) (A and D). Red fluorescence (B and E). Merge (C and F). Transfected cells (as demonstrated by red fluorescence) are indicated by arrows. Bar represents 10 μm.