Table 1 Oligonucleotide primers used for quantitative real time PCR analysis.
From: Expression profiles for six zebrafish genes during gonadal sex differentiation
Primer | Amplicon | Sense primer 5'-3' | Antisense primer 5'-3' |
|---|---|---|---|
ar | 242 nt | AGCAGCAGCACCACTACCA | TTCCTTCCTGCCTCTCGTTC |
sox9a | 719 nt | CGGTGAAGAACGGCCAGAGC | CTGTAGAGTCAGCAATGGGT |
cyp19a1b | 230 nt | AACATTGGACGCATGCATAA | TGTTTGATGGTGCTGATGGT |
dmrt1a | 151 nt | ATGGCAGAGCAGAACGATTT | TAGTCCCACAACAGCATGGA |
fig α | 663 nt | ATGTCGTGTGAAATGACCGGC | CTAGGATGGGAGTGAACTTGG |
cyp19a1a | 131 nt | AGATGTCGAGTTAAAGATCCTGCA | CGACCGGGTGAAAACGTAGA |
β-actin | 272 nt | CCTGACCGAGAGAGGCTACA | CGCAAGATTCCATACCCAAG |