Figure 1
Gene expression of DA receptors D 1,2,4,5 in human GCs. Ethidium bromide-stained agarose gel showing RT-PCR products, which correspond to expected size of D1,2,4,5 cDNAs in GCs (day 3 and day 4 of culture, with and without hCG stimulation). After sequencing they proved to be identical to the human D1,2,4,5. Results of PCR amplification were not visibly different between cells treated with or without hCG, as judged from similar bands and similar levels of cyclophilin, which was also amplified. Control (CO) was without input mRNA in RT-PCR reactions. The gel shows results obtained from one of four experiments that yielded comparable results.