Figure 2From: PKCepsilon and an increase in intracellular calcium concentration are necessary for PGF2alpha to inhibit LH-stimulated progesterone secretion in cultured bovine steroidogenic luteal cellsReduction in PKCε protein. (A) Representative Western blot showing the amount of PKCε and actin expressed in protein samples prepared from luteal steroidogenic cells after 48, 72, and 96 h of transfection with PKCε specific siRNA (lanes 1–3). Lanes labeled 4 – 6, contained protein samples from indicated control treatments (media, Non-sp siRNA, and transfection reagent, respectively). (B) Semi-quantitative analysis of the densitometry derived from four experiments similar to the one shown in panel A. the y-axis shows the ratio of the optical density ratio of PKCε to that of its corresponding β-actin. The data are shown as mean ± SEM, and comparisons were made between different treatments. Values with different letters denote differences by one-way ANOVA followed by Tukey-Kramer honestly significant difference (P < 0.05).Back to article page