Table 1 Primers and RT-PCR conditions for DAX1 isolation and gene expression analysis
Primer | Primer sequences (5'-3') | Thermocycles | Amplicon (bp) |
|---|---|---|---|
TilDax1 | CTGGTGAAGACGGTGCGGTTCGT | 35 cycles: | 587 |
CACTATGGACCCGTGACCTACTT | 94°C 1 min | ||
57°C 1 min | |||
72°C 1 min | |||
sbDax1 | CTGGGGGGTTCTGGTGAAG | 32 cycles: | 161 |
CGGTCTCCGTGGTCTCAAAGTC | 94°C 1 min | ||
59°C 1 min | |||
72°C 1 min | |||
18S | TCAAGAACGAAAGTCGGAGG | 18 cycles: | 450 |
GGACATCTAAGGGCATCACA | 94°C 1 min | ||
59°C 1 min | |||
72°C 1 min | |||
F1 | CTGGCGAAAACTTTCCGATTCCT | ||
F2 | CTCGGCGGTTCTGGTGAAG | ||
F3 | GTGGAGCCCAGCATGCTGCAGC | ||
R1 | CGGTCTCCGTGGTCTCAAAGTC | ||
R2 | AGCCTCCCTGTGAAGTGTCTGG | ||
R3 | CTTCCCGTCACCGGTCTGAAGAAG | ||
R4 | GCCAATGACCGGTTTGAAGAAG |