Table 1 Primers used for gene expression analysis by RT-PCR
Cycle number(N) | ||||||
|---|---|---|---|---|---|---|
Exp1 | Exp2 | |||||
Gene product | Primer Sequence | bp | Ta (°C) | Liv | Tes | Liv |
Estrogen receptor α (ERα, AJ006039) | 5'-CCCATCCAGTCAGCATTCA-3' 5'-TTGTCACGCCGCAGAACG-3' | 374 | 57 | 25 | 25 | 25 |
Estrogen receptor βa (ERβa, AF136980) | 5'-GCTGATGATCGGACTGATGTG-3' 5'-GGTGTACTGTTGGCGGAAAG-3' | 348 | 59 | 27 | 23 | 29 |
Estrogen receptor βb (ERβb, AJ580049) | 5'-TGATGATGTCACTCACCAACC-3' 5'-TTCAGCTCACGAAACCGA-3' | 291 | 54 | 25 | 22 | 26 |
Choriogenin L (ChgL, CX734876) | 5'-AGAGGGATGCTGTCGTAG-3' 5'-GTGATGCCTTTGGTAGTG-3' | 290 | 56 | 25 | 30 | 18 |
Vitellogenin II (VgII, CX734956) | 5'-CACTTGGCATTGGTCTCCC-3' 5'-ATGGTGCACTCAGCTGCATG-3' | 130 | 58 | 20 | 25 | 18 |
18S ribosomal RNA (18S) | 5'-TCAAGAACGAAAGTCGGAGG-3' 5'-GGACATCTAAGGGCATCACA-3' | 495 | 59 | 18 | 18 | 15 |