Figure 5

Expression of integrin subunit mRNA species and drug effects on the expression levels of β ₁ and α ₅ integrin subunits. A. RT-PCR amplification of integrin subunit mRNA species. Transcripts representing integrin subunits were amplified by RT-PCR. Amplicons subjected to Agarose gel electrophoresis, stained with ethidium bromide and photographed. Amplicons representing α_IIb (570 bp), α₂ (541 bp), α_3A(656 bp), α₄ (484 bp), α₅ (564 bp), α_v (619 bp), α₆ (A 420 bp and B 550 bp), β₁ (300 bp), β₃ (200 bp) integrin subunits and β actin (478 bp) transcripts from HTR-8/SVneo cells are shown in lanes 1, 2, 3, 4, 5, 6, 7, 8, 9 and 10 respectively. L: 100 bp molecular weight markers. B. Western blots of integrin subunits β ₁ and α ₅ in control and treated HTR cells. Composite of representative blots showing expression levels of β₁ and α₅ integrin subunits in HTR cells at 3 and 12 h of incubation in absence (control) or presence of low or high concentrations of drugs or alcohol. Integrin subunit bands from two separate experiments (of total four), each consisting of lysate from 3 and 12 h incubation are shown in adjacent lanes. Treatment conditions and integrin subunits are mentioned on the right of each blot. Incubation periods of cells in absence or presence of drugs are shown below the composite.