Table 1 The scale used for evaluation of metallothionein and RCAS1 expression.
Antigen | Immunoreactivity | |||
|---|---|---|---|---|
| Â | 0 | +1 | +2 | +3 |
RCAS1 | No reactivity | Weak (when observed any, also granular in paranuclear region) cytoplasmic staining pattern in up to 10% of positive cells | Marked cytoplasmic (sometimes together with membranous) staining in 11–30% of the cells | High expression – more than 30% of positive cells |
Metallothionein | Lack of any positivity | Weak staining in less than 5% of the cells | Moderate – various staining intensity but in <50% of the cells, | Strong – staining of more than 50% of the cells. |