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Figure 2 | Reproductive Biology and Endocrinology

Figure 2

From: Detection of estrogen receptors ER-alpha and ER-beta in human ejaculated immature spermatozoa with excess residual cytoplasm

Figure 2

Western blotting analysis of ERα and ERβ in human immature and mature ejaculated spermatozoa. A. Immunoblots of sperm extracts by using anti-ERα: a 67 kDa band has been detected in positive control (MCF-7 extract) (lane C+), normal sperm (lane 1) and immature spermatozoa (lines 2–5); the band was lacking in negative control (lane C-). B. Immunoblots of sperm extracts by using anti-ERβ: two bands, 59 and 50 kDa, have been revealed in positive control (LNCaP extract) (lane C+) and immature spermatozoa (lanes 2–5), while normal sperm have shown only the 59 kDa band (lane 1); both the bands were absent in negative control (lane C-). β-actin (in A and B) serves as a loading control.

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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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