Figure 2

Rat chromosomal sequence aligned with Spag11t (A) and Spag11c (B) mRNA and amino acid sequences. Exons are in upper case letters, introns in lower case. Amino acids are indicated in single letters. Numbers in parentheses indicate amino acids of the protein. The gene sequence was extracted from Genbank NW_047475. The rat cDNA sequences for Spag11t and Spag11c are available at Genbank and were assigned the accession numbers AY600144 and AY600145. ^ indicates predicted signal peptide cleavage site. Cysteines are bold underlined. Amino acid sequence shown in italics was cloned and expressed to test their antimicrobial activity. The antibodies were raised against the mouse peptides corresponding to the indicated shaded regions. Furin-like proprotein convertase motif is in bold and underlined. Posttranslational modification sites are indicated: double underlined – N-glycosylation; wavy underlined – casein kinase II phosphorylation; dotted underlined – protein kinase C phosphorylation.