Table 1 Previously reported ICAM-1 immunostaining in non-pregnant and gestational endometrium. FT: first trimester; FF-PE: formalin-fixed, paraffin-embedded; PE: pre-eclampsia, IUGR: intrauterine growth retardation
Reference | Tissue type | Tissue Preparation | Endothelial CellICAM-1 staining | Non-endothelial CellICAM-1 staining |
|---|---|---|---|---|
17 | Cycling endometrium | Frozen (formalin-fix) | Strong, constitutive | Uniform staining of glands, stroma, and epithelium; strong lymphoid staining |
18 | Cycling endometrium | Frozen (formalin-fix) | Strong but variable among vessel types with peak at menstrual | Glandular and luminal epithelium negative, stroma weak in proliferative/secretory phases but strong at menstrual |
19 | Cycling endometrium | Frozen (acetone-fix) and FF-PE | Strong throughout entire cycle | Glandular and luminal epithelium variable, stroma stained throughout cycle with increase expression in menstrual; widespread lymphoid staining |
31 | FT Decidua | Frozen (acetone-fix) | Strong in all vessel types | Glands negative, stroma weak, moderate staining of lymphocytes |
32 | FT Decidua, placenta | Frozen (acetone-fix) | Strong in all vessel types | Stroma negative, strong lymphocyte staining, strong staining of decidua parietalis |
33 | FT decidua | Frozen (acetone-fix) | Strong in all vessel types | Glands negative, some stroma positive |
34 | Decidua, placental bed | Frozen (acetone-fix) | Strong, unchanged in normal vs. PE, IUGR, or PE+IUGR | Weak scattered stromal cell staining |
35 | Decidua, placenta | Frozen (acetone-fix) | Strong, unchanged in normal vs. PE | Villous trophoblasts negative, <10% interstitial trophoblasts stained |
36 | FT decidua | FF-PE | Strong in all vessel types, same in normal vs. inflammation; constitutive | Glands negative, stroma weak |