Table 1 Primer couples and PCR conditions
Gene | Primer sequence D | Primer sequence R | PCR1 | AT2 | CN3 |
|---|---|---|---|---|---|
COX1 | 5'-AAA-TAA-TAT-AAG-CTT-CTG-ACT-CC-3' | 5'-TCC-TAA-AAT-TGA-GGA-AAC-TCC-3' | 190 | 56 | 4.8 |
mtTFA | 5'-CAA-ATG-ATG-GAA-GTT-GGA-CG-3' | 5'-AGC-TTC-CGG-TAT-TGA-GAC-C-3' | 148 | 58 | 6.1 |
NRF1 | 5'-CCC-AAA-CTG-AGC-ACA-TGG-C-3' | 5'-GTT-AAG-TAT-GTC-TGA-ATC-GTC-3' | 162 | 58 | 5.6 |