Table 1 Properties of oligonucleotide primers used for PCR.
From: Extragonadal aromatization increases with time after ovariectomy in rats
Target mRNA | Size of PCR products (bp) | Sense (S) or antisense (AS) | Primer sequence (location) |
|---|---|---|---|
P450c17 | 299 | S AS | 5'-TCATCAAGAAGGGAAAAGAA-3' 5'-TGAAGCAGATAGCACAGATG-3' |
P450arom | 289 | S AS | 5'-GCTTCTCATCGCAGAGTATCCGG-3' 5'-CAAGGGTAAATTCATTGGGCTTGG-3' |
β-actin | 550 | S AS | 5'-AAGCAGGAGTATGACGAGTCCG-3' 5'-GCCTTCATACATCTCAAG TTGG-3' |