Figure 3
From: Selection of gonadotrophin surge attenuating factor phage antibodies by bioassay
Representative example of bioassay testing of scAb/IgG affinity for GnSAF bioactivity. In this case antibody recognition of GnSAF bioactivity in QC hFF was tested compared with GnSAF bioactivity in QC hFF alone (a) or incubated with the non-GnSAF scAb, V peptide (b). Of the 8 individual scAbs tested, 3 showed high affinity for GnSAF, highly significantly reducing GnSAF bioactivity in QC hFF (g, h, i). Data represent the mean of quadruplicate determinations from 2 different rat pituitary cell culture bioassays. The shaded dose-response curves (a-j) indicates the mean ± SE range for QC hFF incubated with culture medium (a, open circles). The significance value (by ANOVA) indicates the significance of reduction of GnSAF bioactivity.