Figure 2
From: Selection of gonadotrophin surge attenuating factor phage antibodies by bioassay
Initial screening of pooled scAb populations. The Dyematrex Blue A process left GnSAF bioactivity in the unbound fraction (a) and this was used to pan the phage display library. Neither anti-M13 (closed circles) nor SFDM (open circles) affected GnSAF bioactivity levels in QC hFF (b). ScAbs recognising the Dyematrex Blue A bound fraction and a non-GnSAF protein (V peptide) also had no affinity for GnSAF bioactivity (c, e) while those recognising the unbound Dyematrex Blue A fraction significantly reduced GnSAF bioactivity in QC hFF. Data represent the mean of quadruplicate determinations from 2 different rat pituitary cell culture bioassays. The shaded horizontal bar (a) indicates the mean ± SE range for control GnRH-induced LH secretion while the shaded dose-response curves (b-e) indicates the mean ± SE range for QC hFF incubated with culture medium (b, open circles). The significance value (by ANOVA) indicates the significance of reduction of GnSAF bioactivity.