Figure 4
Expression of TGFβRII in cultured granulosa cells. Determination of expression of TGFβRII in granulosa cells immediately following collection and following 48 hours of culture. Lanes 1–3 contain PCR products (766 bases) following amplification with ovine TGFβRII primers from 3 separate pools of granulosa cells at the time of collection, lanes 4–6 contain PCR products (766 bases) following amplification with ovine TGFβRII primers from 3 separate pools of granulosa cells collected 48 hours after culture, lane 7 contains the negative control water blank whereas lane 8 contains the PCR product from the positive control 4 week old ovary sample. Migration of DNA molecular weight standards are indicated on the left hand side.