Figure 9
Plasma proteins, 20 μg per lane, from Arctic char treated with 17-β-estradiol (E2, 10-7 M) or/and cortisol (F, 10-5 M) separated on 8% SDS-PAGE. Coomassie-stained gel and corresponding Western blot using a polyclonal antibody against Arctic char vitellogenin. Lane 1: control, lane 2: E2, lane 3: F, lane 4: E2 + F. Molecular weight (Da) are shown to the left.