Strategies for the production of genetically identical monkeys by embryo splitting

Table 1 Developmental competence in vitro of split monkey embryos following aggregation of individual diploid and tetraploid blastomeres from 4-cell stage embryos

	Developmental Stage (%)
Treatment Group^a,b,c	n	Compacted	Blastocyst	Cell #
Quarter (1 diploid blastomere)	47	48.6a	17.4a	48.7a
Half (2 diploid blastomeres)	53	59.5a	39.8b	81.4b
Half Chimera (1 diploid and 1 tetraploid blastomere)	49	65.9a,b	43.2b	94.1b
Control (nonmanipulated embryos)	41	88.0b	59.6b	165.7c

^aQuarter = single blastomere from a 4-cell stage diploid embryo; Half = two blastomeres from a 4-cell stage diploid embryo; Half Chimera = single blastomere from a 4-cell stage diploid embryo and a single blastomere from a 4-cell stage tetraploid embryo; Control = intact nonmanipulated diploid embryos; Cell # = numbers of nucleated cells in blastocysts on Day 7 post insemination. ^bn = total number of embryos (7 replicates) ^cDifferent letters within columns denote significant (P < .05) differences among treatments.

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