Expression of connexins in human preimplantation embryos in vitro

Table 4 Summary of staining human blastocysts with anti-connexin antibodies

Embryo	Cx43	Cx26	Cx31	Cx45	Cx40
1	+	+
2	+	+
3	+	+
4	+	+
5	-	-
6	+	+
7	+		-
8	+		+
9	-		-
10	-		-
11	+		-
12	+			+
13	+			+
14	+			+
15	+			+
16	-			-
17	+			-
18	+				-
19	+				-
20	+				-
21	-				-
22	-				-
23	+				-

Individual human blastocysts were stained with antibodies to Cx 43,26, 31, 45 and 40 as indicated in materials and methods. Double staining with Cx43 and one other connexin was carried out in each case. +, specific staining detected; -, no staining detected.

ISSN: 1477-7827

Embryo	Cx43	Cx26	Cx31	Cx45	Cx40
1	+	+
2	+	+
3	+	+
4	+	+
5	-	-
6	+	+
7	+		-
8	+		+
9	-		-
10	-		-
11	+		-
12	+			+
13	+			+
14	+			+
15	+			+
16	-			-
17	+			-
18	+				-
19	+				-
20	+				-
21	-				-
22	-				-
23	+				-

Embryo	Cx43	Cx26	Cx31	Cx45	Cx40
1	+	+
2	+	+
3	+	+
4	+	+
5	-	-
6	+	+
7	+		-
8	+		+
9	-		-
10	-		-
11	+		-
12	+			+
13	+			+
14	+			+
15	+			+
16	-			-
17	+			-
18	+				-
19	+				-
20	+				-
21	-				-
22	-				-
23	+				-

Embryo	Cx43	Cx26	Cx31	Cx45	Cx40
1	+	+
2	+	+
3	+	+
4	+	+
5	-	-
6	+	+
7	+		-
8	+		+
9	-		-
10	-		-
11	+		-
12	+			+
13	+			+
14	+			+
15	+			+
16	-			-
17	+			-
18	+				-
19	+				-
20	+				-
21	-				-
22	-				-
23	+				-