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Figure 5 | Reproductive Biology and Endocrinology

Figure 5

From: Fundulus heteroclitus gonadotropins.5: Small scale chromatographic fractionation of pituitary extracts into components with different steroidogenic activities using homologous bioassays

Figure 5

Mono-P fractions #I-V (containing Polybuffer) were readjusted with start buffer [1.2 M (NH4)2SO4 in 0.1 M sodium phosphate, pH 6.8]. Each individual Mono-P fraction was rechromatographed on a Phenyl-5-PW column. A linear gradient of decreasing salt concentration was started after the Polybuffer eluted. Shaded areas indicate fractions containing gonadotropic activities (see Table 1).

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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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