Figure 7

Immuno-localization of germ cell markers in oocyte-like structures from young mice (A and C) and aged mice (B and D). The oocyte-like structures stained positive in young mice (A and C) and aged mice (B and D) for VASA (A and B) and GDF-9 (C and D) in postcultured OSE. These markers are specific to ooplasm, and surrounding fibroblasts were negative. Oocyte-like structure (O) which surrounded zona pellucid (ZP)-like structure and cumulus (C)-like cells is prominently observed in young mice. DAB (3, 3’-diaminobenzidine)-HRP substrate was used to produce a brown reaction in immunohistochemical analysis. Scale bar = 20 μm.