Figure 3
From: SET protein up-regulated testosterone production in the cultured preantral follicles
Effect of SET in the regulation of steroidogenic enzyme expression in theca cells. The cultured follicles were infected with recombinant adenoviruses for 24 h. The expression of SET, StAR, CYP11A1, CYP17A1 and HSD3B2 were analyzed by qRT-PCR. GAPDH were used as internal controls. (A) Compared to those receiving AdCMV, the SET mRNA of AdCMV-SET infected follicles was significantly increased and the expression of CYP17A1 and HSD3B2 were elevated. (B) The SET mRNA of AdSiRNA-SET infection group was significantly diminished compared with AdSiRNA-NS and the mRNA levels of CYP17A1 and HSD3B2 decreased significantly. Results were presented as Mean ± SD from at least 3 independent experiments. Sample sizes were 20 follicles per treatment, per experiment. *, p < 0.05 compared with the control adenoviruses infected group.