Table 1 Primers used in real-time quantitative PCR of genes in goose samples
Gene name | Accession number | Prime sequences (5′ to 3′) | Annealing temperature (°C) | PCR product (bp) |
|---|---|---|---|---|
LHR | U31987.1 | upstream: CTCTGTGATAACTTGCGTAT | 52.8 | 119 |
downstream: AAGGCATGACTGTGGAT | 52.8 | 279 | ||
FSHR | NM_205079.1 | upstream: CAACCTTCCCAAACTAC | 52.8 | 327 |
downstream: ATTTCCTGAATCCCATT | ||||
ß-Glycan | M77809.1 | upstream: GTGAACTTCCCAATAGCA | ||
downstream: AGTGTCCAGACCGTAGAAC | ||||
Activin IIRα | D31899.1 | upstream: ACTGACTTCCTCAAGGCTAA | 52.8 | 131 |
downstream: GAGATGGCGGGTTTATGT | 55.8 | 114 | ||
Inhibin α | NM_001031257.1 | upstream: GGGCTGGAAGAGGTAGGTGAAGAT | 55.8 | 113 |
downstream: GGAGGACACGTCGCAGGTGAT | ||||
Inhibin ßA | NM_205206.1 | upstream: CAGTGGACACCCGAAAGA | ||
downstream: GCACAGGTCACAGGCAAT | ||||
Activin IR | NM_204560.1 | upstream: TGCCTGGATGGTTTCGTC | 55.8 | 189 |
downstream: GCCATTTCTCATCGGGACT | ||||
Activin IIRβ | NM_204317.1 | upstream: ATTTACTACAACGCCAACT | 55.8 | 143 |
downstream: CAGCCTTTCTTCACCAG | ||||
β-Actin | L08165.1 | upstream: GAGAGAAATTGTGCGTGA | 55.8/52.5 | 195 |
downstream: TCCATACCCAAGAAAGAT |