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Figure 5 | Reproductive Biology and Endocrinology

Figure 5

From: Sperm-associated antigen 11A is expressed exclusively in the principal cells of the mouse caput epididymis in an androgen-dependent manner

Figure 5

Western blots analyses confirmed the presence of SPAG11A protein in the epididymis. Fifteen micrograms of protein from each region of the epididymis was separated by 10% SDS-PAGE and transferred to PVDF membranes. SPAG11A was detected with a rabbit anti-human SPAG11A antibody. IS = initial segment; Cap = Caput; Cor = Corpus; Cau = Cauda. The results showed the presence of SPAG11A (MW = 20 kDa, indicated by an arrow) in the mouse caput epididymis. A very weak and smear band of SPAG11A was detected in the corpus, whereas no signals were observed in the initial segment and cauda. The same membrane was stripped and re-probed with antibody against α-tubulin as a loading control.

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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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