Figure 1

In silico analyses of Spag11a sequence. (A) The gene structure of Spag11a consists of two exons (113 and 322 bp) separated by a single 1257 bp intron. The shaded areas indicate the UTR. The start codon (ATG) is located in the middle of exon 1, and the stop codon is at the end of exon 2. (B) The Spag11a transcript is 435 bp long with the start codon (ATG) located at nucleotide 59 (n59, horizontal arrow), the stop codon (TAG) at n266 (asterisk sign) and 5′ and 3′ untranslated regions (UTR, shaded area). The translation of Spag11a produces 69 residues with the first 20 amino acids (a1-20) constituting a signal peptide (underlined). The downward triangle indicates a cleavage site at position a20 (D: aspartate). Amino acids 26–61 (dotted underline) contain a beta defensin domain, and a24-29 (greater than signs, >>>) contain an N-myristoylation site. A protein kinase C phosphorylation site is located at a64-66 (double underlined), which overlaps with a casein kinase II phosphorylation site at a64-67 (less than signs, <<<). The boundary between exon 1 and exon 2 is indicated by a down arrow.