Table 3 Differentially expressed proteins in ROS- and ROS+ groups along with the spectral count and the spectral count ratio for the two groups
From: Proteomic analysis of human spermatozoa proteins with oxidative stress
Protein | NCBI # | SC ROS- | NSC-ROS- | SC ROS+ | NSC-ROS+ | NSC ratio in ROS+/ROS- |
|---|---|---|---|---|---|---|
acetyl-Coenzyme A acetyltransferase 1 precursor (ACAT1) | 4557237 | 20 | 0.006 | 26.000 | 0.008 | 1.4 |
acid phosphatase, prostate short isoform precursor (ACPP) | 6382064 | 17 | 0.005 | 27.000 | 0.009 | 1.7 |
actin, alpha 1, skeletal muscle (ACTA1) | 4501881 | 0 | 0.000 | 8.000 | 0.003 | ROS + only |
A-kinase anchor protein 3 (AKAP3) | 21493041 | 76 | 0.022 | 70.000 | 0.022 | 1.0 |
A-kinase anchor protein 4 isoform 2 (AKAP4) | 21493039 | 497 | 0.145 | 467.000 | 0.147 | 1.0 |
angiotensin I converting enzyme 1 isoform 1 precursor (ACE) | 4503273 | 5 | 0.001 | 5.000 | 0.002 | 1.1 |
ATP synthase, H + transporting, mitochondrial F1 complex, alpha subunit precursor (ATP5A1) | 4757810 | 28 | 0.008 | 30.000 | 0.009 | 1.2 |
beta actin (ACTB) | 4501885 | 53 | 0.015 | 36.000 | 0.011 | 0.7 |
brain creatine kinase (CKB) | 21536286 | 19 | 0.006 | 21.000 | 0.007 | 1.2 |
chaperonin containing TCP1, subunit 4 (delta) (CCT4) | 38455427 | 4 | 0.001 | 6.000 | 0.002 | 1.6 |
chaperonin containing TCP1, subunit 5 (epsilon) (CCT8) | 24307939 | 4 | 0.001 | 7.000 | 0.002 | 1.9 |
chaperonin containing TCP1, subunit 8 (theta) | 48762932 | 5 | 0.001 | 4.000 | 0.001 | 0.9 |
chromosome 20 open reading frame 3 (C20orf3) | 24308201 | 2 | 0.001 | 4.000 | 0.001 | 2.2 |
clathrin heavy chain 1 (CLTC) | 4758012 | 14 | 0.004 | 6.000 | 0.002 | 0.5 |
clusterin preproprotein (CLU) | 355594753 | 56 | 0.016 | 55.000 | 0.017 | 1.1 |
CSE1 chromosome segregation 1-like protein (CSE1L) | 29029559 | 3 | 0.001 | 0.000 | 0.000 | ROS- only |
enolase 1 (ENO1) | 4503571 | 7 | 0.002 | 2.000 | 0.001 | 0.3 |
eukaryotic translation elongation factor 1 alpha 1 (EEF1A1) | 4503471 | 28 | 0.008 | 33.000 | 0.010 | 1.3 |
eukaryotic translation elongation factor 2 (EEF2) | 4503483 | 12 | 0.003 | 5.000 | 0.002 | 0.5 |
fatty acid synthase (FASN) | 41872631 | 40 | 0.012 | 35.000 | 0.011 | 1.0 |
fibronectin 1 isoform 3 preproprotein (FN1) | 16933542 | 41 | 0.012 | 21.000 | 0.007 | 0.6 |
fructose-bisphosphate aldolase A (ALDOA) | 4557305 | 15 | 0.004 | 17.000 | 0.005 | 1.2 |
fumarate hydratase precursor (FH) | 19743875 | 5 | 0.001 | 7.000 | 0.002 | 1.5 |
glutamine synthetase (GLUL) | 19923206 | 4 | 0.001 | 12.000 | 0.004 | 3.2 |
glutathione peroxidase 4 isoform A precursor (GPX4) | 75709200 | 8 | 0.002 | 21.000 | 0.007 | 2.8 |
glutathione S-transferase mu 3 (GSTM3) | 23065552 | 50 | 0.015 | 65.000 | 0.020 | 1.4 |
glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | 7669492 | 14 | 0.004 | 22.000 | 0.007 | 1.7 |
glyceraldehyde-3-phosphate dehydrogenase, spermatogenic (GAPDHS) | 7657116 | 210 | 0.061 | 185.000 | 0.058 | 1.0 |
heat shock 70Â kDa protein 2 (HSPA2) | 13676857 | 26 | 0.008 | 22.000 | 0.007 | 0.9 |
heat shock 70Â kDa protein 5 (HSPA5) | 16507237 | 5 | 0.001 | 11.000 | 0.003 | 2.4 |
heat shock 90Â kDa protein 1, alpha isoform 1 (HSP90AA1) | 153792590 | 39 | 0.011 | 26.000 | 0.008 | 0.7 |
heat shock protein 90Â kDa beta, member 1 (HSP90B1) | 4507677 | 11 | 0.003 | 22.000 | 0.007 | 2.2 |
heat shock protein beta-1 (HSPB1) | 4504517 | 5 | 0.001 | 4.000 | 0.001 | 0.9 |
histone cluster 1, H2aa (HIST1H2AA) | 25092737 | 47 | 0.014 | 56.000 | 0.018 | 1.3 |
histone cluster 1, H2ae (HISTH1AE) | 10645195 | 14 | 0.004 | 13.000 | 0.004 | 1.0 |
histone cluster 1, H2ba (HIST1H2BA) | 24586679 | 22 | 0.006 | 49.000 | 0.015 | 2.4 |
lactotransferrin precursor (LTF) | 54607120 | 246 | 0.072 | 292.000 | 0.092 | 1.3 |
lectin, mannose-binding 2 precursor | 5803023 | 3 | 0.001 | 5.000 | 0.002 | 1.8 |
L-lactate dehydrogenase A isoform 1 (LDHA) | 5031857 | 10 | 0.003 | 9.000 | 0.003 | 1.0 |
L-lactate dehydrogenase C (LDHC) | 4504973 | 17 | 0.005 | 12.000 | 0.004 | 0.8 |
mitochondrial ATP synthase beta subunit precursor (ATP5B) | 32189394 | 50 | 0.015 | 70.000 | 0.022 | 1.5 |
mitochondrial malate dehydrogenase precursor (MDH2) | 21735621 | 22 | 0.006 | 44.000 | 0.014 | 2.2 |
olfactomedin 4 precursor (OLFM4) | 32313593 | 1 | 0.000 | 4.000 | 0.001 | 4.3 |
outer dense fiber of sperm tails 1 (ODF1) | 194248060 | 15 | 0.004 | 14.000 | 0.004 | 1.0 |
outer dense fiber of sperm tails 2 isoform 2 (ODF2) | 24430183 | 90 | 0.026 | 91.000 | 0.029 | 1.1 |
peptidylprolyl isomerase A (PPIA) | 10863927 | 4 | 0.001 | 2.000 | 0.001 | 0.5 |
peroxiredoxin 6 (PRDX6) | 4758638 | 4 | 0.001 | 1.000 | 0.0003 | 0.3 |
phosphoglycerate dehydrogenase (PHGDH) | 23308577 | 3 | 0.001 | 5.000 | 0.002 | 1.8 |
phosphoglycerate kinase 2 (PGK2) | 31543397 | 17 | 0.005 | 9.000 | 0.003 | 0.6 |
phospholipase A2, group IIA precursor (PLA2G2A) | 4505849 | 6 | 0.002 | 10.000 | 0.003 | 1.8 |
prolactin-induced protein (PIP) | 4505821 | 118 | 0.034 | 101.000 | 0.032 | 0.9 |
prostate specific antigen isoform 1 preproprotein (KLK3) | 4502173 | 3 | 0.001 | 2.000 | 0.001 | 0.7 |
protein disulfide-isomerase A3 precursor (PDIA3) | 21361657 | 7 | 0.002 | 7.000 | 0.002 | 1.1 |
pyruvate kinase, muscle isoform M2 (PKM) | 33286418 | 49 | 0.014 | 31.000 | 0.010 | 0.7 |
RAB2A, member RAS oncogene family (RAB2A) | 4506365 | 3 | 0.001 | 3.000 | 0.001 | 1.1 |
ropporin (ROPN1) | 21359920 | 20 | 0.006 | 31.000 | 0.010 | 1.7 |
ropporin, rhophilin associated protein 1B (ROPN1B) | 59891409 | 73 | 0.021 | 73.000 | 0.023 | 1.1 |
saccharopine dehydrogenase (putative) (SCCPDH) | 55770836 | 13 | 0.004 | 16.000 | 0.005 | 1.3 |
semenogelin I isoform a preproprotein (SEMG1) | 4506883 | 171 | 0.050 | 101.000 | 0.032 | 0.6 |
semenogelin II precursor (SEMG2) | 4506885 | 513 | 0.149 | 226.000 | 0.071 | 0.5 |
sorbitol dehydrogenase (SORD) | 156627571 | 36 | 0.010 | 27.000 | 0.009 | 0.8 |
sperm acrosomal membrane protein 14 | 19424138 | 1 | 0.000 | 2.000 | 0.001 | 2.2 |
sperm autoantigenic protein 17 (SPA17) | 8394343 | 15 | 0.004 | 20.000 | 0.006 | 1.4 |
sperm protein associated with the nucleus, X chromosome, family member C (SPANX) | 13435137 | 54 | 0.016 | 80.000 | 0.025 | 1.6 |
transglutaminase 4 (prostate) (TGM4) | 156627577 | 4 | 0.001 | 9.000 | 0.003 | 2.4 |
triosephosphate isomerase 1 isoform 1 (TPI1) | 4507645 | 28 | 0.008 | 31.000 | 0.010 | 1.2 |
tubulin alpha 6 (TUBA1C) | 14389309 | 9 | 0.003 | 11.000 | 0.003 | 1.3 |
tubulin, alpha 3c (TUBA3C) | 17921993 | 117 | 0.034 | 134.000 | 0.042 | 1.2 |
tubulin, beta 4 (TUBB4A) | 21361322 | 36 | 0.010 | 30.000 | 0.009 | 0.9 |
tubulin, beta, 2 (TUBB4B) | 5174735 | 232 | 0.068 | 235.000 | 0.074 | 1.1 |
tyrosine 3/tryptophan 5 -monooxygenase activation protein, zeta polypeptide (YWHAZ) | 4507953 | 4 | 0.001 | 3.000 | 0.001 | 0.8 |
valosin-containing protein (VCP) | 6005942 | 16 | 0.005 | 9.000 | 0.003 | 0.6 |
voltage-dependent anion channel 2 (VDAC2) | 42476281 | 11 | 0.003 | 11.000 | 0.003 | 1.1 |
voltage-dependent anion channel 3 isoform b | 25188179 | 7 | 0.002 | 12.000 | 0.004 | 1.9 |