Figure 3

Maternal diabetes induces ER redistribution defects during in vivo oocyte maturation (GV and Pro-MII stages). GV and Pro-MII oocytes collected from control and diabetic mice were labeled with ER Tracker Green to visualize ER localization. ER distribution patterns were evaluated using fluorescence microscopy. A, in fully grown GV oocytes, three distinct patterns of ER distribution were detected: homogeneous distribution pattern (i), ER clouds (white arrows) (ii), and cluster distribution patterns (iii); B, in Pro-MII oocytes, three different ER distribution patterns were identified: perinuclear distribution pattern (i), homogeneous distribution (ii), and clustering distribution (iii). Bar = 20 μm.